Louise and Martine Fokkens, 70, have finally decided they are too old for the business after claiming to have slept with 355,000 men between them in a combined 100-year career.
Louise, a mother of four, has said her arthritis now makes some sexual positions 'too painful'.
Ladies in red: The twins, who usually dress in matching clothing, became prostitutes at the age of 20
Game changer: After 50 years selling sex, Louise and Martine Fokkens are giving up prostitution
She said: 'I couldn't give him up. He's been coming to me for so long it's like going to church on a Sunday.'
The pair were the subject of a documentary film last year called Meet the Fokkens, and have now written a book about their combined 100-years of sexual exploits called The Ladies of Amsterdam.
Both women - who usually dress in identical red clothes - became prostitutes before the age of 20 to survive financially after escaping violent relationships.
They now look back on the 'golden years' of the profession before the Netherlands legalised prostitution and the sex trade was invaded by 'eastern European mafia'.
Louise said: 'It is very different now. We used to sit in the windows with clothes on. Today they are totally naked.
'There are few Dutch women and no sense of community these days.'
Martine added: 'The legalisation of brothels in 2000 has not improved prostitutes' lives.
Double act: Louise said she was forced to retire after arthritis made some sexual positions 'too painful'
Laid bare: The twins are hoping to survive from the book and films rights to the documentary about their lives called Meet the Fokkens